Thesis

An Immunohistochemical Study of the Endometrial Expression of Angiogenic Growth Factors and Their Corresponding Receptors

The human endometrium is essential to female reproductive function. Endometrium, the inner mucous lining of the uterus, constitutes the surface where the fertilized egg implants.

Immunohistochemistry is the technique where substances, such as growth factors, in sections of tissue are localized by use of specific antibodies. This technique has been central throughout my PhD studies.

Angiogenesis, the new building of blood vessel from existing ones (Folkman, 1985).

There are numerous interesting angiogenic growth factors discovered. Below, some of those I have studied a bit closer are listed.

1. Vascular endothelial growth factor (VEGF-A,B,C, and D)

2. Fibroblast growth factor 2 (FGF-2)

3. Epidermal growth factor (EGF)

I have used image analysis to quantify immunohistochemical stainings. Additionally, by use of immunohistochemical stainings of vessels performed stereological analysis.

I have worked with SDS-page in order to detect angiogenic peptides in endometrial tissue. Additionally to verify the function and specificity of different antibodies.

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